PP-306 An adipose derived decellularised extracellular matrix as a biomaterial for tissue engineering of soft tissue

Published Date: 17th July 2023

Publication Authors: Yong L

Introduction
Vascularised tissue transplants are currently used to reconstruct soft tissue defects caused by trauma, cancer or infection but is not without its limitations and risks. Decellularised extracellular matrix are tissues that have been processed to remove cellular content whilst retaining its network of structural proteins, glycoproteins and adhesion proteins, providing a natural scaffold for cellular attachment, migration and tissue formation, making it a suitable candidate biomaterial for tissue engineering vascularised tissues.

Methods
Using the method developed by Poon et al (1), we created a decellularised extracellular matrix hydrogel from porcine adipose tissue (pDAT) by applying physical and chemical processing methods to remove lipids and cellular content. DNA from the pDAT and native porcine tissue was extracted using the Qiagen DNeasy kit and quantified using the NanoDrop. Nuclei was visualised with DAPI staining. Liquid chromatography‐coupled tandem mass spectrometry was used to characterise the proteome of the decellularised matrix compared to native tissue. Characterisation of tissue generation, angiogenic and immune properties in vivo was assessed using the rodent flow through model(2) and compared against gelatin methacrylate 7.5% w.t. (Gelma), a mixture of Gelma 7.5% w.t. and pDAT, pDAT alone, a commercially available decellularised matrix (Jellagen), as well as a self‐assembling synthetic peptide (Manchester Biogel Alpha 2).

Results
We successfully produced a hydrogel with thermal gelling properties. DNA quantification showed a statistically significant decrease in DNA content, with corresponding DAPI staining showing no nuclei staining. Proteomic data shows the presence of various collagens including collagen I and IV as well as extracellular matrix proteins such as fibronectin and laminin remaining in pDAT. Tissue generation was greater in both the decellularised matrixes. The number of blood vessels per area was greatest in the Gelma/pDAT mixture. Jellagen appears to induce the greatest foreign body response.

Discussion & Conclusions
pDAT shows promise as a biomaterial for tissue regeneration with potential for use in tissue engineering.

 

Yong, L et al. (2023). PP-306 An adipose derived decellularised extracellular matrix as a biomaterial for tissue engineering of soft tissue. Tissue Engineering Part A. 29(13 and 14). [Online]. Available at: https://doi.org/10.1089/ten.tea.2023.29043.abstract [Accessed 21 December 2023]

 

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