Urinary proteomics

Published Date: 19th July 2016

Publication Authors: Hardy KJ

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Introduction

Obstructive sleep apnoea (OSA) is a common complication of obesity and can have a substantial negative impact on a patient's quality of life and risk of cardiovascular disease. In this study, discovery profiling of urinary peptides was performed by capillary electrophoresis- mass spectrometry (CE-MS) in obese subjects with and without obstructive sleep apnoea without a history of coronary artery disease.

Methods

Obese subjects with and without OSA were recruited from weight management clinics. Anthropometry, blood pressure and fasting blood samples were taken for measurement of metabolic syndrome components. Overnight polysomnography was conducted to confirm or refute OSA. Urinary samples were analysed by CE-MS. OSA patients were naive to continuous positive airway pressure treatment.

Results

61 OSA subjects (age 47 + 9years, BMI 42.6 + 8.6kg/m²); 31 controls (age 49 + 10years, BMI 39.3 + 5.9kg/m²) were studied; p = ns for age and BMI. Apnoea-hypopnoea Index was higher in OSA patients (24 + 18.6) than controls (2.6 + 1.1;p < 0.001). Metabolic syndrome was present in 35(38%) OSA vs 4(13%) controls; p <0.05). 24 polypeptides were found to be candidates for differential distribution (p < 0.01), although these differences did not reach significance when multiple testing was accounted for. Sequences were determined in 8 peptides demonstrating origins from collagens and fibrinogen alpha.

Conclusion

In this study, we report for the first time, urinary proteomic profile analyses using CE-MS in OSA and non-OSA obese groups. The differences in urinary proteomic profiles prior to adjustment for multiple testing, with increased metabolic syndrome in obese OSA subjects, suggests the potential for such methods for identifying obese patients at high risk for sleep apnoea.

Seetho, I; Hardy, K et al. (2014).  Urinary proteomics in obstructive sleep apnoea with obesity . Obesity Facts. 7 (Suppl 1), 12.

Seetho, IW; Hardy, KJ; et al. (2014).  Urinary proteomics in obstructive sleep apnoea and obesity . European Journal of Clinical Investigation. 44 (11), p1104-1115​

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